Inhibition by Berberine and Cytosine Deaminase Enzyme Suicide Gene on Rectum Cancer Cells
- VernacularTitle:黄连素联合胞嘧碇脱氨酶自杀基因对直肠癌细胞的抑制作用
- Author:
Xiongwen ZHU
;
Xueqiang MA
;
Bin LIANG
- Publication Type:Journal Article
- Keywords:
Rectum cancer;
Berberine;
Suicide gene
- From:
Journal of Medical Research
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the killing effect by berberine and cytosine deaminase enzyme suicide gene on rectum cancer cells in vitro.Methods Human rectum cancer HR-8348 cells were cultured,then transfected by recombinant adenovirus suicide gene the survivial percentage of which was examined by MTT method.Results Recon of pcDNA3.1(+)-CD was constructed successfully.The CD gene sequence analysis indicated th a+ recon pcDNA3.1(+)-CD contain intact gene.RT-PCR showed CD gene expressed stably in the HR-8348 cells,which is sensitive to 5-FC above the density of 100?g/ml.Elevated along with the medicine density with response time lengthening,its damaging effect enhanced.However for the density above 5-FC 250g/ml,the change of cell killing rate was not apparent.when by 0.1,0.3,3.0,30.0?m density berberine unites the 5-FC on rectum cancer of the intestines Respectively,the cell inhibitory action elevates along with density rises,but 3.0?m,30?m the inhibition change was not apparent.Under 250?g/ml of 5-FC,0.1,0.3,3.0,30.0?m of berberine united the 5-FC The HR-8348 cells growth suppression rate were:27.7%,42.4%,52.3%,56.3% respectively.Conclusion Inhibition function to human rectum cancer HR-8348 cells can be strengthened by berberine combined 5-FC.
