Anti-fibrosis effect and mechanism of total flavones of Bidens bipinnata L on immunological liver fibrosis in rats
- VernacularTitle:鬼针草总黄酮抗大鼠免疫性肝纤维化作用及部分机制研究
- Author:
Fanrong WU
;
Feihu CHEN
;
Wei HU
;
Fenglai YUAN
;
Liping YUAN
;
Jianhua WANG
;
Jun LI
- Publication Type:Journal Article
- Keywords:
total flavones of Bidens bipinnata L(TFB);
liver fibrosis;
pig serum
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the anti-fibros is effect and mechanism of total flavones of Bidens bipinnata L(TFB) on immunological liver fibrosis in rats.Methods The pig serum was injected into abdominal cavity(0.5 ml,twice a week) for 12 weeks so as to induce hepatic fibrosis,and then the rats were treated with TFB daily for 10 weeks and killed at the 22 nd week.The HA,LN,PCⅢ and CⅣ in serum were assessed by ELISA.Liver samples collected after experiment were stained with hematoxylin-eosin(HE) staining,Masson staining and scored.The expression of ?-smooth muscle actin(?-SMA) in liver was analyzed by immunohistochemistry.The gene expression of TGF-?1 was detected by RT-PCR.Results Compared with model group,TFB treatment significantly reduced HA,LN,PCⅢ,CⅣ content in serum.Liver histology in the TFB treated rats was also improved.Moreover TFB could decrease the expression of protein ?-SMA and TGF-?1 mRNA.Conclusions TFB significantly reduced pig serum-induced liver fibrosis in rats,probably by decreasing the expression of TGF-?1 mRNA and then inhibiting the proliferation of HSC.
