Neuroprotection effects of exogenous adrenomedullin against hypoxic-ischemia reperfusion brain damage in neonatal rats
- VernacularTitle:肾上腺髓质素对新生大鼠缺氧缺血再灌注性脑损伤的保护作用
- Author:
Shifa ZHANG
;
Mingxiong ZHOU
;
Changsheng DOU
;
Benbiao GUO
- Publication Type:Journal Article
- Keywords:
hypoxia-ischemia,brain;
adrenomedulin;
nitricoxide synthase;
glutathione;
peroxydase;
rat
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the neuroprotection effects exogenous adrenomedullin(AM) against hypoxic-ischemia reperfusion damage(HIRBD) in neonatal rat models.METHODS:Forty neonatal rats were randomly divided into Control Group(n=8,with sham operation),Hypoxic-ischemia Reperfusion(HIR) Group and Intervention Group(n=8,wth intraperitoneal AM injection 0,6 and 12 h after making model).All rats were decapitated at 48 hours after reperfusion,and the cerebral slices were made at the 2 mm level in front of the interaural line.The pathobiological changes of brain tissue were observed by the method of HE dye in light microscope,and the levels of nitrogen monoxide synthase(NOS),glutathion(GSH) and myeloperoxidase(MPO) in neonatal rats cerebral tissue were determined by using chromatometry.RESULTS:Hypoxic-ischemia reperfusion could induce obviously cerebral pathological change which could be improved by AM.The brain tissue levels of cNOS and GSH in AM 0 and 6 h Group were significantly higher than those of HIR Group(P
