Role of glucose transporters in apoptosis induced by sodium butyrate
- VernacularTitle:葡萄糖转运蛋白在丁酸钠诱导结肠癌细胞凋亡中的作用
- Author:
Xi LI
;
Hesheng LUO
- Publication Type:Journal Article
- Keywords:
Butyrates;
Glucose transporter;
Apoptosis
- From:
Chinese Journal of Practical Internal Medicine
2006;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The regulation on glucose transporters(GLUT1~GLUT5)by sodium butyrate was observed,and the regulation on bax and bcl-x/l by sodium butyrate and glucose was studied.This helped to learn about the possible mechanism of apoptosis of cancer cells induced by sodium butyrate.Methods The expression of GLUT1~GLUT5 mRNA was detected by RT-PCR.The expression of bax and bcl-x/l was detected by immuocytochemistry.Cell apoptosis was detected by TUNEL assay.Results In HT-29 cell line,sodium butyrate downregulated the expression of GLUT1 mRNA and the expression of bcl-x/l and induced cancer cell apoptosis,but did not regulate the expression of GLUT2,GLUT3,GLUT5 and bax.GLUT4 was not detected in HT-29 cell line.And when treated with sodium butyrate,the increase of concentration of glucose could increase the expression of bcl-x/l and reduce the effect of apoptosis induced by sodium butyrate.Without treatment with sodium butyrate,the change of glucose concentration only had a feeble regulation on apoptosis.Conclusion Sodium butyrate can obviously downregulate the expression of GLUT1 and downregulation of the expression of GLUT1 is associated with the apoptosis induced by sodium butyrate in HT-29 cell line.
