Role of protein kinase C-?(PKC?) in GSTT-induced cardioprotection
- VernacularTitle:蛋白激酶C Eplison亚型在蒺藜皂苷诱导心脏保护中的作用
- Author:
Wei SUN
;
Hong LI
;
Hongmei YU
;
Shijie YANG
- Publication Type:Journal Article
- Keywords:
myocardial reperfusion injury;
GSTT;
PKC?;
chele
- From:
Chinese Pharmacological Bulletin
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the mechanism of protection of reperfused ischemic heart by Gross Saponin Tribulus Terrestris(GSTT).Methods Hearts of 40 SD rats were isolated,linked to Langendorff perfusion apparatus,and randomly divided into 5 equal groups:control group,to be perfused with modified Kreb-Henseleit(K-H) buffer for 170 min;ischemia /reperfusion(I/R) group,to be perfused with K-H buffer for 20 min,exposed to ischemia for 30 min,and then reperfused with K-H buffer for 120 min;Chelerythrine group,to be perfused with K-H buffer with Chelerythrine,a PKC? antagonist for 20 min,exposed to ischemia for 30 min,and then reperfused with K-H buffer with Chelerythrine for 120 min;GSTT group,perfused with K-H buffer with GSTT for 20 min,exposed to ischemia for 30 min,and then reperfused with K-H buffer with GSTT for 120 min;and GSTT+Chelerythrine group,perfused with K-H buffer with GSTT+Chelerythrine for 20 min,exposed to ischemia for 30 min,and then reperfused with K-H buffer with GSTT+Chelerythrine for 120 min.The heart rate,mean aortic pressure and CK-MB were measured 20 min after the stabilization of perfusion,and 60 or 120 min after reperfusion.After the stop of reperfusion,hearts were sliced into 1-mm-thick transverse sections and incubated in triphenyltetrazolium chloride solution(TTC) to determine infarct size.The phosphorylation level and translocation of PKC? from hearts were determined by western blot analysis.Results GSTT significantly reduced infarct size from 63.2%?4.7% in controls to 37.2%?15.7%.And GSTT could reinforce PKC? translocate from cytoplasm to cellular membrane.Chelerythrine abolished these effects.Conclusion GSTT protects the heart from I/R injury partially by activating PKC?.
