HPLC Determination of Berberine Hydrochloride in GuZhiNing Liniment
- VernacularTitle:HPLC法测定骨质宁搽剂中盐酸小檗碱的含量
- Author:
Shifang XU
;
Aiying CHEN
- Publication Type:Journal Article
- Keywords:
GuZhiNing,Berberine Hydrochloride,assay,HPLC
- From:Journal of Zhejiang Chinese Medical University
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To develop an HPLC method for the determination of berberine hydrochloride in GuZheNing liniment.[Methods]The chromatographic system consisted of Dimonsil C18 column and mobile phase of acetonitrile—50mmol?L-1 sodium dihydrogen phosphate(adjusted PH to 2.5 with H3PO4)(25∶75).The flow rate was 1.0 mL?min-1,and the detective wavelength was at 348 nm.[Results]The calibration curve was linear in the range of 0.106～0.530 ?g(n=5).The method was proved to be repeatable with RSD 0.7%(n=6).4 batches of sample were analyzed and the Berberine Hydrochloride contents were 19.11,18.81,18.83,18.92mg/100mg,respectively.[Conclusion]The method is selective,simple and accurate,and could be used for the quality control of this preparation.
