THE GROWTH INHIBITION AND APOPTOSIS-INDUCING EFFECTS OF APIGENIN IN HUMAN GASTRIC CARCINOMA CELLS
- VernacularTitle:芹菜素对人胃癌细胞的生长抑制和凋亡诱导作用的研究
- Author:
Linhong YUAN
;
Wei XIA
;
Xiujuan ZHAO
;
Xiaohua ZHANG
;
Kun WU
- Publication Type:Journal Article
- Keywords:
apigenin;
human gastric cancer cell;
apoptosis;
caspase-3;
Bcl-2
- From:
Acta Nutrimenta Sinica
1956;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the growth inhibition and apoptosis-inducing effects of apigenin in human gastric cancer SGC-7901 cells. Method: MTT and flowcytometry were used to detect the growth inhibition and cell cycle distribution in apigenin-treated SGC-7901 cells respectively. DAPI fluorescence staining and DNA ladder assay were applied to study the pro-apoptosis effects of apigenin. The expression of apoptosis relative proteins, caspase-3 and Bcl-2, were analyzed by using Western blotting. Results: Apigenin treatment significantly inhibited the growth of human gastric cancer cell SGC-7901 and markedly caused their apoptosis following activation of caspase-3 and downregulation of Bcl-2 protein expression. Conclusion: The activation of caspase-3 and downregulation of apoptosis relative protein Bcl-2 expression were the possible mechanism of apigenin induced growth inhibition and apoptosis in SGC-7901 cells.
