Total flavones of Bidens pilosa L′s effect on cytokines in liver fibrosis rats
- VernacularTitle:鬼针草总黄酮对肝纤维化大鼠细胞因子的影响
- Author:
Liping YUAN
;
Feihu CHEN
;
Lijuan XIA
;
Mingmei ZHONG
;
Lei ZHANG
;
Jun LI
- Publication Type:Journal Article
- Keywords:
total flavones of Bidens pilosa L(TFB);
liver fibrosis;
transforming growth factor-?1 (TGF-?1);
NF-?B
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To study the effect of total flavones of Bidens pilosa L (TFB) on cytokines production in liver fibrosis rats. Methods Rat liver fibrosis was induced by CCl450%, 0.1 ml?(100 g)-1 body weight twice a week for 18 weeks. TFB (160,80,40 mg?kg-1) was used daily via gastrogavage at 9 week. Levels of TNF-? and IL-1? in serum were determinate by radioimmunoassay. Liver samples were collected after experiments and stained by immuninochemistry of TGF-?1 and NF-?B. Moreover TGF-?1 mRNA expression in liver tissue was detected by RT-PCR technology. Results TFB (160, 80 mg?kg-1) could significantly reduce serum TNF-? and IL-1? contents; TFB(160, 80, 40 mg?kg-1) could effectively prevent the expression of NF-?B,as was TGF-?1 of TFB(160, 80 mg?kg-1). Moreover TFB (160, 80 mg?kg-1) could significantly reduce TGF-?1 mRNA in liver fibrosis rats. Conclusion TFB had protective effect on liver fibrosis by its inhibition of cytokine production.
