Roles of hypoxia regulated genes/protein in neuroprotection against hypoxia injury in differentiated SH-SY5Y cells preconditioned with CoCl_2
- VernacularTitle:缺氧调节基因/产物参与CoCl_2预处理对分化SH-SY5Y细胞缺氧损伤的保护
- Author:
Sheng SUN
;
Wenxiang GAO
;
Yuqi GAO
- Publication Type:Journal Article
- Keywords:
hypoxia regulated genes;
chemical hypoxia preconditioning;
hypoxia inducible factor;
human neuroblastoma SH-SY5Ycells;
neuroprotection
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the roles of hypoxia regulated genes/protein in chemical hypoxia preconditioning in differentiated SH-SY5Y cells.Methods Differentiated SH-SY5Y cells were randomly divided into control group,chemical hypoxic preconditioning group(50 ?mol/L CoCl2 preconditioning for 3 h,normal culture for 1 h,then in 2%O2 for 28 h) and hypoxia group(in 2%O2 for 28 h).RT-PCR was used to examine the mRNA expressions of VEGF,GLUT-1,EPO,LDH-A.Further evaluation of the potential neuroprotective effect of VEGF was done by investigating the effect of recombinant human VEGF on subsequent hypoxia injury.Results The mRNA levels of GLUT-1,EPO increased in the preconditioning group as compared with those in the hypoxia group(P
