Bioinformatic analysis of the cellular repressor of E1A-stimulated genes proximal promoter and primary identification of transcriptional regulation function
- VernacularTitle:人E1A激活基因阻遏子基因启动子生物信息学分析及转录调控功能初探
- Author:
Yaling HAN
;
Xin ZHAO
;
Chenghui YAN
- Publication Type:Journal Article
- Keywords:
Promoter;
Bioinformatics;
wtp53;
Repressor protein;
E1A;
Phenotype
- From:
Chinese Journal of Practical Internal Medicine
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the human cellular repressor of E1A-stimulated genes (hCREG)using bioinformatics tools and predict the promoter position and transciption factor binding sites.Methods Complete coding hCREG sequences were obtained from Genbank.hCREG was analyzed with First EF software in order to obtain the promoter sequence of hCREG.Moreover,transcription factor binding sites of hCREG was convinced by Motif software.Subsequently,the transcription factor of hCREG by bioinformatics tools was related between hCREG and smooth muscle cells differentiation observed by both Western blot and immunoflourescence.Results The promoter of hCREG was located in -109~-359 bp of up-stream of transcriptional site,which was predicted with 945bp length.Transciption factor binding sites of hCREG was predicted by Motif software which was found with 80 transciption factors.The expression of hCREG and smooth muscle ?-actin(SM ?-actin)increased in HITASY after 72 hours with serum deprivation detected by both Western blot and immunoflourescence.Meanwhile,the results showed that the expression of wtp53 increased significantly.These results suggested that transcription factor wtp53 might regulate the expression of hCREG and promoted dedifferentiated phenotype of VSMCs.Conclusion The transcriptional information of the proximal promoter of hCREG obtained.As up-stream regulational factor of hCREG,wtp53 can up-regulate the expression of hCREG and may play a vital role in the process of VSMCs differentiation and phenotype modulation.
