Expression of p38MAPK and TGF?_2 in retina of experimental diabetic hamster
- VernacularTitle:糖尿病仓鼠视网膜p38MAPK和TGF?_2的表达
- Author:
Hui PENG
;
Suling HONG
;
Yongxian TAO
;
Yan PENG
;
Rong JIANG
- Publication Type:Journal Article
- Keywords:
diabetic retinopathy;
RT-PCR;
Western blotting;
p38MAPK signal channel;
diabetic hamster
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of p38MAPK and TGF?2 in the development of diabetic retinopathy.Methods Fifteen Hamsters were induced into diabetic models by intraperitoneal injection of Streptozotocin(40 mg/kg once a day) for 3 d and 13 Hamsters were successfully established,whose blood glucose was over 13.5 mmol/L.Ten Hamsters as controls were intraperitoneally injected of physical saline of the same volume.At 16th week after induction,the total RNA of retina from all sacrificed Hamsters was collected.The mRNA expressions of p38MAPK,TGF?2 in retina were detected by semi-quantitative RT-PCR and their protein levels by Western blotting.Results The mRNA expressions of p38MAPK,TGF?2 in retina were of high tendency and their protein levels increased.Conclusion p38MAPK signal pathway may involve in the pathogenesis of diabetic retinopathy.
