Inhibitory effect of receptor of advanced glycation endproducts,nuclear factor-?B double gene antisense RNA on productions of inflammatory factor treated by advanced glycation endproducts
- VernacularTitle:晚期糖化终产物受体、核因子-?B双基因反义RNA抑制糖化终产物刺激的炎症因子表达
- Author:
Jie YOU
- Publication Type:Journal Article
- Keywords:
antisense RNA;
advanced glycation endproducts;
receptor;
nuclear factor-?B;
gene transfection;
inflammatory factor
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effects of receptor of advanced glycation endproducts(RAGE),NF-?B double gene antisense RNA on the productions of TNF-? and IL-6 treated by advanced glycation endproducts(AGEs).METHODS:The levels of TNF-? and IL-6 in the supernatants were measured by enzyme linked immunosorbent assay(ELISA) in ECV304 cells treated by AGEs.The RAGE,NF-?B single/double gene antisense RNA were transfected into ECV304 cell.The expressions of RAGE and NF-?B were detected by flow cytometry and RT-PCR. In different clone cells,the effects of antisense RNA on the productions of TNF-?、IL-6 were determined by ELISA.RESULTS:AGEs,instead of human serum albumin(HSA),stimulated ECV304 cell to produce TNF-? and IL-6 with a time-and dose-dependent manner.The RAGE,NF-?B single/double gene antisense RNA were transfected into ECV304 cell.Induced by AGEs,the expressions of RAGE and NF-?B in double gene cotransfected cell were inhibited by((62.2)?(8.7))% and((37.2)?(7.1))%,respectively.Induced by AGEs,the amount of TNF-?、IL-6 in the medium were lower in single gene transfected cells ECV-asRAGE,ECV-asP65 than ECV-Vector(P
