Determination of glutamate in microamount cerebrospinal fluid after acute brain injury in mice
- VernacularTitle:小鼠急性脑损伤后微量脑脊液谷氨酸浓度的测定
- Author:
Wei LI
- Publication Type:Journal Article
- Keywords:
acute cerebral insult;
microamount;
cerebrospinal fluid;
glutamate
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To establish a method for determining glutamate concentration in microamount cerebrospinal fluid after acute cerebral insult.Methods The concentration of glutamate in cerebrospinal fluid was measured in mice after acute cerebral insult by precolumn derivatization with O-phthaldehyde.Results The average recovery of glutamate was 84.26% to 125.6%(n=3),the relative standard deviation(RSD) in one day and three days was respectively less than 4.94%(n=3),0.78%(n=3).The concentration of glutamate in cerebrospinal fluid of mice 6 h after acute cerebral insult was significantly higher than that of sham operation mice(P
