Effect of D-gal on mouse fibroblast telomere shortening
- VernacularTitle:D-gal对鼠成纤维细胞端粒长度的影响
- Author:
Hang XIAO
- Publication Type:Journal Article
- Keywords:
cell;
senescence;
telomere
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify that the length of mice fibroblast telomere can be shortened by D-gal.Methods The fibroblasts were from the heart tissue and passaged for 3-5 generations.After the fibroblasts were mixed with(C_(3)TA_(2))_(3)PNA labeled with FITC,the variation of fibroblast telomere length was detected by Flow-Fish.Results The length of fibroblast telomere can be shortened by D-gal.Conclusion D-gal can lead to the fibroblast telomere shortening.It demonstrates that D-gal can cause cellular senescence,which establishes the foundation for the senescence model of heart fibroblast.
