The inhibition of genistein on proliferation and transcription of c-fos mRNA in human umbilical vascular smooth muscle cells induced by MCP-1
- VernacularTitle:金雀异黄素对MCP-1诱导人脐静脉血管平滑肌细胞增殖及c-fos mRNA转录的影响
- Author:
Xiumei GUAN
;
Zanjie FENG
;
Minzhang QIAN
- Publication Type:Journal Article
- Keywords:
genistein;
MCP-1;
c-fos gene;
atherosclerosis
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the inhibition of genistein on proliferation and transcription of c-fos mRNA in human umbilical vascular smooth muscle cells(hUVSMC) induced by monocyte chemotactic protein-1(MCP-1). Methods Growth-arrested hUVSMC were stimulated with MCP-1(10 ?g?L-1) prior to co-treatment with different concentrations of genistein (10,30,90 ?mol?L-1). The response of hUVMSC to these treatments was observed in comparison with that of control group. The proliferation of hUVMSC was evaluated by cell counting. The expression of c-fos mRNA was detected by RT-PCR. Results Low concentration of genistein(10 ?mol?L-1) inhibited the proliferation of hUVSMC and high concentration of genistein(30,90 ?mol?L-1) inhibited the expression of c-fos in hUVSMC induced by MCP-1. Conclusions Genistein could suppress the proliferation of hUVSMC induced by of MCP-1. Its mechanisms may involve the down-regulation of c-fos mRNA expression.
