Design of livin antisense oligonucleotides with computer
- VernacularTitle:计算机辅助设计抗肿瘤凋亡基因livin的反义核酸
- Author:
Huadong ZHANG
;
Shoujun YUAN
;
Huipeng CHEN
- Publication Type:Journal Article
- Keywords:
the secondary structure of mRNA;
RNAdraw;
Sfold;
livin antisense ODNs
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: Antisense oligonucleotides against livin were designed with computer software. METHODS: Antisense oligonucleotides were designed according to the secondary structure of livin mRNA which was simulated with RNAdraw and Sfold. And then these oligonucleotides were transfected into Hela cells for inducing apoptosis. RESULTS: Five antisense oligonucleotides were designed using RNAdraw and Sfold, and effectively induced Hela cell apoptosis. CONCLUSION: The approach is effective and feasible to design antisense oligonucleotides by means of calculation with two kinds of software.
