Effects of crocetin on doxorubicin-induced damage in rats with myocardial mitochondria
- VernacularTitle:西红花酸对阿霉素所致大鼠心肌线粒体损伤的影响
- Author:
Wenna LI
;
Zhiyu QIAN
- Publication Type:Journal Article
- Keywords:
crocetin;
doxorubicin;
mitochondria;
cytochrome C oxidase subunit II (COII)
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2004;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the protective effects of crocetin on the doxorubicin-induced damage in rats with myocardial mitochondria. METHODS: Rats were given intraperitoneal injection of doxorubicin to induce cardiotoxicity. After continuous oral administration of crocetin, the rats were sacrificed, and myocardial mitochondria were isolated. The mitochondrial membrane potential (MMP), O~-_2. production, the activity of respiratory chain complex IV and glutathione peroxidase (GSH-PX), and DNA fragmentation were determined. The expression level of COII gene was determined through RT-PCR. RESULTS: Crocetin increased the activity of respiratory chain complex IV and GSH-PX, MMP and the expression level of COII and decreased DNA fragmentation and superoxide anion radical O~-_2. production in rats with myocardial mitochondria. CONCLUSION: Crocetin may decrease the damage in rat myocardial mitochondria induced by doxorubicin.
