Biodistribution characteristics of ~(99m)Tc labeled anti-colon cancer monoclonal antibody in mice
- VernacularTitle:~(99m)Tc标记的大肠癌单克隆抗体在小鼠体内分布特点的研究
- Publication Type:Journal Article
- Keywords: mTc-ACCmAb; colon cancer; distribution; SPECT radio-imaging
- From: Chinese Journal of Clinical Pharmacology and Therapeutics 2004;0(07):-
- CountryChina
- Language:Chinese
- Abstract: AIM: To study the biodistribution of 99m Tc labeled Anti-colon cancer monoclonal antibody ( 99mTc-ACCmAb ) in normal and nude mice and the imaging of same antibody in nude mice, which were carried with human colon cancer. METHODS: Normal mice and Nude mice that are carried the L010-16 human colon cancer received, intravenously, 99m Tc labeled monoclonal antibodies, and then the radioactivity was detected in normal mice in different time points and the imaging was checked by using the SPECT in nude mice. RESULTS: The radioactivity of 99m Tc at 6 h 5 min decreased 76.6% in comparison with the 30 min in normal mice blood, and the radioactivity was the 23.4% of the 30 min in the blood. The radioactivity in 6 h 5 min was 27.2% of the 30 min in normal mice liver and decreased 76.8%. In the normal mice injected 99m Tc-ACCmAb, the radioactivity at 6 h 5 min decreased 68.4% compared with the 30 min in normal mice blood, the radioactivity was the 31.6% of the 30 min in the blood, the radioactivity at 24 h are 6.1% and it dropped 93.9% compare with the 6 h 5 min. In nude mice, the radioactivity of 99m Tc-ACCmAb at 24 h is 2.1% of the first half-life. It decreased 97.9% compared with the 6 h 5 min. Although the ratio of blood and tumor was the highest, the imaging results were still better than other time points. CONCLUSION: 99m Tc-ACCmAb may concentrate in nude mice, which are carried with human colon carcinoma. It may become the diagnostic reagent for radio-immunoguided and ensure to remove the tumor during operations.