EFFECT OF GENISTEIN ON THE EXPRESSION OF UPA AND THE ACTIVITY OF PTK IN MDA-MB-453 BREAST CANCER CELLS
- VernacularTitle:染料木黄酮对MDA-MB-453乳腺癌细胞uPA表达及PTK活性的影响研究
- Author:
Xiaoping YU
;
Mantian MI
;
Jundong ZHU
- Publication Type:Journal Article
- Keywords:
genistein;
urokinase-type plasminogen activator;
protein tyrosine kinase;
MDA-MB-453 breast cancer cell
- From:
Acta Nutrimenta Sinica
1956;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of genistein on the expression of urokinase-type plasminogen activator (uPA) and the activity of protein tyrosine kinase (PTK) in MDA-MB-453 cells, and explore the molecular mechanism of anti-angiogenesis in HER-2/neu-overexpressing breast cancer by genistein. Methods:Western blot, immunoprecipitate, reverse transcription-polymerase chain reaction (RT-PCR) and kinase activity analysis technics were used to observe the expression of uPA and the protein phosphorylation of HER-2/neu receptor and the activity of protein tyrosine kinase (PTK) in MDA-MB-453 cells treated by genistein for 24, 48, 72 h. Results: The expression of uPA and the protein phosphorylation of HER-2/neu receptor and the activity of PTK were significantly decreased after treated with 5?10-5mol/L genistein, which had a time-dependence. Conclusion: Genistein could inhibit the activity of PTK and the protein phosphorylation of HER-2/neu receptor, and down-regulate the exprssion of uPA at transcription and translation levels in breast cancer cells. This might be a part of molecular mechanism of genistein anti-angiogenesis in HER-2/neu-overexpressing breast cancer.
