Screening and identification of proteins that interact with receptor for advanced glycation end products (RAGE) via T7 select phage display system
- VernacularTitle:用T7噬菌体展示系统筛选与晚期糖基化终产物受体胞内段相结合的蛋白
- Author:
Shanchao ZHAO
;
Zhijie LI
;
Jinghua LIU
- Publication Type:Journal Article
- Keywords:
glycosylation end products, advanced;
receptors;
binding sites;
bacteriophage T7
- From:Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen and identify proteins that interact with receptor for advanced glycation end products (RAGE) with T7 select phage display system. Method GST-tagged fusion protein of RAGE was coated on glutathione suporflow resin and binding screens were performed with human lung T7 phage library. Results After 6 rounds of biopanning, 64 independent plaques were scraped and processed with EDTA. The inserted fragments in these plaques were amplified by PCR and the PCR products were purified by a gel recovery method. The sequences of the insertions were identified and analyzed with BLAST program in GeneBank. Twenty-five clones of encoding proteins were found. Conclusions T7 select phage display system is a convenient, rapid and effective method for screening binding proteins. This study provides an approach to explore complicated functions and novel mechanisms of RAGE-related signal transduction pathways.
