EFFECTS OF PRO-INFLAMMATORY MEDIATORS ON ADVANCED GLYCATION END PRODUCT RECEPTORS IN TYPE-B SYNOVIAL CELLS
- VernacularTitle:促炎症介质对B型滑膜细胞晚期糖基化终产物受体的调节作用
- Author:
Jianping JIANG
;
Fanfan HOU
;
Xun ZHANG
- Publication Type:Journal Article
- Keywords:
synovial cells;
advanced glycosylation end products;
receptors;
regulation
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
To elucidate the effects of pro inflammatory mediators on mRNA expression of AGE receptors in type B synovial cells, type B synovial cells from normal subjects were isolated and cultured in vitro with human serum albumin modified with advanced glycation end products (AGE HSA), tumor necrosis factor ?(TNF ?)and interleukin 1?(IL 1?). The expression of RAGE mRNA and AGE R3 mRNA was examined by RT PCR techniques. TNF ?, AGE HSA and IL 1? up regulated the expression of AGE R3 mRNA in type B synovial cells in a dose and time dependent manner. In contrast, TNF ? and AGE HSA down regulated the expression of RAGE mRNA in a dose and time dependent manner. IL 1? had no effect on RAGE mRNA expression. The regulatory responses induced by AGE HSA were blocked by a neutralizing polyclonal anti human TNF ? antibody, suggesting that the effects of AGE HSA were mediated by TNF ?. The proinflammatory mediators may regulate the gene expression of AGE receptors in type B synovial cells, and the regulatory role of these receptors is different in response to the proinflammatory mediators.
