Expression of advanced glycation end products receptors on human joint synovial cells
- VernacularTitle:人类关节滑膜细胞表达晚期糖基化终产物受体
- Author:
Fanfan HOU
;
Jianping JIANG
;
Xun ZHANG
- Publication Type:Journal Article
- Keywords:
Synovial cells;
Advanced glycation end products;
Receptor
- From:
Chinese Journal of Nephrology
1997;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the expression of advanced glycation end products(AGE) binding proteins on human joint synovial cells for elucidating the pathobiological effects of ?2m modified with AGE(AGE-?2m) on joint resident cells. Methods Type A and type B synovial cells were isolated and cultured in vitro. The expression of AGE receptor 1 (ACE-R1 ), AGE receptor 2 (AGE-R2), AGE receptor 3 (AGE-R3) and 35 KD receptor for AGE(RAGE) on synoviocytes were detected by immunofluorescent staining using specific antibodies and flow cytometric analyses. mRNA of AGE receptors was examined by RT-PCR techniques.-Results RAGE and AGE-R3, but not AGE-R1 and AGE-R2, were constitutively expressed on the membrane surface of both type A and type B synovial cells. These two types of synovial cell also expressed mRNA of RAGE and AGE-R3. Conclusion Human joint synovial cells express specific AGE binding proteins, RAGE and AGE-R3, suggesting that these cells may be involved in AGE metabolism and might be the target of the biological effects of AGEs in dialysis-related amyloidosis.
