The Potential Alteration of Their Osteogenesis and Adipocyte Differentiation in Bone Marrow Mesenchymal Stem Cells from Mice with Immune-mediated Aplastic Anemia
- VernacularTitle:免疫介导型再生障碍性贫血小鼠间充质干细胞成骨及成脂潜能改变
- Author:
Ruilan GAO
;
Liming YIN
;
Xudai QIAN
;
Zhou HANG
- Publication Type:Journal Article
- Keywords:
aplastic anemia;
mesenchymal stem cell;
osteogenesis potential;
adipogenic potential
- From:
Journal of Zhejiang Chinese Medical University
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore the potential alteration of their osteogenesis and adipocyte differentiation in bone marrow mesenchymal stems cells from mice with immune-mediated aplastic anemia.[Methods]Balb/c mice model of immune-mediated aplastic anemia was established by radiation with sublethal dose of 60Co following the intravenously infusion lymphocytes of DAB/2 mice.The culture of the MSC and CFU-F and pathological examination of bone marrow were carried out 15 days later.The amount of calcium node and the frequency of adipocyte differentiation were evaluated respectively by alizarin red and oil red O.[Result]The number of CFU-F and the number of calcium node in model mice decreased more greatly than normal mice,but the ferenqucy of adipocyte differentiation was more increased greatly in model mice than normal mice;the pathological examination showed in the model mice,the hematopoietic structure was destroyed and filled with abundant adipocyte.[Conclusion]The potention of osteogenesis and adipocyte differentiation was altered in the mice with immune-mediated aplastic anemia.
