Protective effects of adeno-associated viral gene transfer of BDNF on the hippocampal neurons
- VernacularTitle:表达大鼠脑源性神经营养因子腺相关病毒(rAAV-BDNF)对体外培养海马神经元保护作用的研究
- Author:
Jingyu ZHANG
;
Jiexu ZHAO
- Publication Type:Journal Article
- Keywords:
rAAV-BDNF;
Apoptosis;
Gene therapy
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effective mechanism for transfection of rat brain derived neurotrophic factor(BDNF) gene by recombinant adeno-associated virus in the cultured hippocampal neurons.Methods:rAAV-BDNF was injected into the serum free-induced neurons.DAPI,PI and Actin stain was performed to measure the apoptosis cells and morphogenesis of the cells.Results:After the gene transfer,the survival rate of the serum free-induced neurons was increased by 65%.Conclusion:The recombinant adeno-associated virus vector can protect the serum free-induced neurons from Apoptosis.
