Effects of genistein on the matrix metalloproteinase inhibitor 1 mRNA levels of HSC-T6 cell in vitro
- VernacularTitle:木黄酮对体外培养的HSC-T6细胞基质金属蛋白酶抑制因子1基因表达的影响
- Author:
Yun WANG
;
Junwang XU
;
Lei FU
- Publication Type:Journal Article
- Keywords:
liver cirrhosis;
estradiol;
phytoestrogen genistein;
matrix metalloproteinase inhibitor;
hepatic stellate cell
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective By using estradiol(E2) as positive control to observe the effects of genistein(GST) on the matrix metalloproteinase inhibitor 1 mRNA levels of HSC-T6 cell in vitro.Methods HSC-T6 cells were exposed to different concentrations of E2 0.1?mol/L and GST 0.5-50?mol/L for 36 hours.The TIMP-1 mRNA levels were measured by the quantitative reverse-transcription polymerase chain reaction(RT-PCR).Results The TIMP-1 mRNA levels of HSC-T6 cells at different concentrations of E2 and GST were lower than those of the normal control(P
