Establishment of genetic idiopathic hypercalciuric rats model
- VernacularTitle:遗传特发性高钙尿大鼠模型的建立
- Author:
Shaogang WANG
;
Dongxi LUO
;
Jihong LIU
- Publication Type:Journal Article
- Keywords:
Hypercalciuria;
Animal model;
Vitamin D receptor
- From:
Chinese Journal of Urology
2006;0(S2):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a colony of genetic idiopathic hypercalciuric rats model which can stablely descend and initially explore the mechanism of idiopathic hypercalciuric development. Methods The male and female rats with the highest 24-h urine Ca excretion and normal serum calcium,phosphorus and 1,25(OH)2D3 were chosen for inbreeding to propagate the colony until the stable hypercalcinuria was achieved in filial generation. Immunohistochemical determination (SP method) was undertaken to determine the VDR expression in model rats duodenum. Results The content of two 24 h urine Ca excretion in 93% male and 92% female generation 7 model rats was double standard deviation more than that in the normal group(2.56?0.86 mg/d vs. 1.12?0.18 mg/d,2.86?1.16 mg/d vs. 1.15?0.12 mg/d respectively)(F=27.10,P*0.05). VDR expression was remarkably increased in model rats duodenum(F=14.23,P
