The effect of interleukin-2 (IL-2) synergized with IL-18 to enhance activities of natural killer cells
- VernacularTitle:白细胞介素-2协同白细胞介素-18活化自然杀伤细胞活性的作用
- Author:
Ping SONG
;
Chengyun ZHENG
- Publication Type:Journal Article
- Keywords:
interleukin-2;
interleukin-18;
natural killer cell;
myeloma cells
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore potential synergetic effects of interleukin-2(IL-2) with IL-18 on induction of natural killer(NK) cell activity against myeloma cells in vitro.Methods CD69 expression on NK cells were defined by Flow Cytometry;IFN-? level in supernatants of NK cell culture was measured by ELISA;pure NK cell population was obtained from peripheral blood by using cell purification kit;standard Cr51 release method was used to test NK cell cytotoxicity against myeloma cells in vitro.Results The combined application of IL-18 with low dose of IL-2(10u/mL) significantly enhanced NK cell activation,IFN-? secretion and NK cell cytolytic activity against myeloma cells(U266 and RPMI-8226) as compared to IL-18 alone,respectively.Conclusion IL-2 synergized with IL-18 to induce NK cells activates anti-myeloma cell in vitro and combined use of IL-18 and low dose IL-2 could be an alterative immuno-therapy approach for cancers including myeloma.
