Role of Apoptosis-Related Gene survivin, caspase-3 and cyclin-B1 in Gastric Carcinoma Tumorigenesis
- VernacularTitle:凋亡相关基因survivin、caspase-3及cyclin-B1在胃癌发生、发展中的作用
- Author:
Yayuan WEN
;
Baohua LIU
;
Shenglong HONG
;
Tao FU
- Publication Type:Journal Article
- Keywords:
Gastric carcinoma Gene Apoptosis
- From:
Chinese Journal of Bases and Clinics in General Surgery
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of apoptosis-related gene survivin, caspase-3 and cyclin-B1 in gastric carcinoma by detecting the expressions of survivin, caspase-3 and cyclin-B1 in gastric carcinoma. Methods The expressions of survivin mRNA, caspase-3 mRNA and cyclin-B1 mRNA were determined by reverse transcription-polymerase chain reaction (RT-PCR) method in 30 gastric carcinoma specimens and 10 normal gastric tissue specimens. Results The positive expression rate of survivin mRNA in 30 gastric carcinoma specimens was 66.7%(20/30). While 10 normal gastric tissue specimens did not express survivin mRNA. Although all of 30 gastric carcinoma tissues and 10 normal gastric tissues expressed caspase-3 mRNA and cyclin-B1 mRNA, the expressions of caspase-3 and cyclin-B1 in 20 survivin-positive gastric carcinoma tissues were significantly lower than those of 10 survivin-negative gastric carcinoma tissues (P
