The effect of transplantation of skeletal muscle satellite cell on retard the denervated muscles atrophy
- VernacularTitle:骨骼肌卫星细胞移植对延缓失神经肌肉萎缩的作用
- Author:
Zhiqiang ZHAO
;
Qiang LIU
;
Gang LI
- Publication Type:Journal Article
- Keywords:
Satellite cells, skeletal muscle;
Muscle atrophy;
Transplantation
- From:Chinese Journal of Orthopaedics
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect which the skeletal muscle satellite cells were transplanted on denervated skeletal muscle atrophy so as to provide the experimental data for treating denervated muscle atrophy in vivo. Methods Thirty-two Sprague-Dawley rats weighting 200-250 g were randomly divided into control group and experimental group. Each group included 16 rats, the animal model of denervated gastrocnemius muscles were formed by cutting the right sciatic nerve of rats caused nerve despair about 1 cm. Muscle satellite cells were obtained from the dorsal and lower exterenity muscle of SD rats. Before transplantation, muscle satellite cells being labeled with DAPI (4′-6-Diamidino-2-Phenylin Dole) in vitro. Muscle satellite cells and NS were implanted into denervated skeletal muscle from the two groups. Bilateral gastrocnemius muscles of each rat from the two groups were taken and weighed at second and eighth post-operative weeks respectively. The above muscles underwent anti-actin immunohistochemical and HE staining and muscle cross-sectional area of fiber and the actin content of the rats were measured by the image analysis, the data of which was handled with SPSS software. Results The satellite cells and myofiber with fluorescence were observed in transplantation site. The group of skeletal muscle satellite cells transplanted when two weeks and eight weeks denervated gastrocnemius muscle wet weight remnant rate and muscle cross-sectional areas of fiber remnant rate and muscle actin content were better than in the control group respectively(P
