The cloning and expression of an anti-Alzheimer's disease neuroprotective peptide gene humanin
- VernacularTitle:抗阿尔茨海默病神经保护肽humanin基因的克隆与表达
- Author:
Benyan LUO
;
Min YUAN
;
Min TANG
;
Zhi CHEN
- Publication Type:Journal Article
- Keywords:
Proteins;
Recombinant fusion proteins;
Alzheimer disease;
Cloning,molecular;
Gene expression
- From:
Chinese Journal of Neurology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To use gene engineering technique to express the humanin.Methods Humanin segment was synthesized and cloned into the prokaryocyte expression vector pGEMEX-1 and pet44a. The plasmid was transformed to E. coli BL21(DE3) by screening and identifying to obtain the high expressing positive clone.Results The humanin segment was cloned into vector successfully,and expressed efficiently with the inducement of IPTG. The molecular weight of the expressed recombinant protein of pGEMEX-1-humanin was about 28 kd while it was 63 kd in pet44a-humanin,and the expressed quantity of fusion protein was 40 percentage of the total bacterial protein while it was 20 percent in pet44a-humanin.Conclusion The humanin has been cloned and expressed successfully and the high efficiently expressed recombinant protein might contain the intact important structure domain of the humanin.
