Effects of advanced glycation end products on activi ty and expression of matrix metalloproteinase-2 in renal cortex of rats
- VernacularTitle:糖基化终末产物对大鼠肾皮质基质金属蛋白酶2活性和表达的影响
- Author:
Xiaoyan YU
;
Cai LI
;
Ze HE
;
Chunsheng MIAO
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathies;
Glycosylation e nd products, advanced;
Gelatinase A (Matrix metallproteinase-2);
Tissue inhibi tor of metalloproteinase-2
- From:
Chinese Journal of Endocrinology and Metabolism
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To in ve stigate the effects of advanced glycation end products (AGEs) on matrix metallop roteinase-2 (MMP-2) activity and its mRNA expression in renal cortex of rats. Methods Diabetic model of rats was induced by s treptozotocin. AGEs were prepared by incubation of rat serum protein with 0.5 mo l/L glucose. AGEs was administered intravenously to normal rats (AGEs group), an d native rat serum protein was given as negative control (negative group) and no rmal rats without treatment were as control (control group). AGEs content in ren al cortex and serum was quantified by ELISA, MMP-2 and TIMP-2 mRNA expressions were examined by RT-PCR and MMP-2 activity was measured by zymography. Results AGEs content increased significantly, MMP-2 mRNA expression descended and TIMP-2 mRNA expression ascended in renal cortex o f diabetic rats (all P
