Content Determination of Chlorogenic Acid in Different Dosage Forms of Qingkailing Preparation by RP-HPLC
- VernacularTitle:RP-HPLC法测定不同清开灵剂型中绿原酸的含量
- Author:
Xiaoling CAI
;
Yiqiang QIN
;
Jungang DENG
- Publication Type:Journal Article
- Keywords:
Qingkailing;
Capsule;
Granules;
Dripping pills;
Chlorogenic acid;
RP-HPLC;
Content determination
- From:
China Pharmacy
2007;0(31):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish RP-HPLC method for the content determination of chlorogenic acid in 3 kinds of dosage forms of Qingkailing preparation. METHODS: SinoChrom ODS-AP C18(250 mm?4.6 mm, 5 ?m) column was adopted and the mobile phase consisted of methnol-water-acetic acid (25 ∶ 75 ∶ 0.5) at the flow rate of 1.0 mL?min-1. Column temperature was set at 30 ℃ and the detection wavelength was set at 327 nm. RESULTS: The linear range of chlorogenic acid was 3.81～152.50 ?g?mL-1(r=0.999 8), and the average recoveries of the capsules, granules and dripping pills were 99.03%(RSD=2.02%), 99.35%(RSD=1.16%),98.11%(RSD=1.51%). CONCLUSION: The method is sensitive, simple and accurate for the quality control and content determination of 3 kinds of Qingkailing preparations.
