Anti-tumor Activity of Adriamycin-loaded Chitosan Nanoparticles Surface-modified with Glycyrrhizin on Human Hepatoma Cell Line SMMC-7721
- VernacularTitle:甘草酸修饰阿霉素壳聚糖纳米粒对人肝癌细胞系SMMC-7721的杀伤作用研究
- Author:
Aihua LIN
- Publication Type:Journal Article
- Keywords:
Glycyrrhizin;
Adriamycin;
Chitosan nanoparticles;
SMMC-7721 cell line;
Active drug delivery system;
Anti-tumor activity
- From:
China Pharmacy
2007;0(31):-
- CountryChina
- Language:Chinese
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Abstract:
OBJECTIVE: To investigate the anti-tumor activity of adriamycin-loaded chitosan nanoparticles surface-modified with glycyrrhizin on human hepatoma cell line SMMC-7721. METHODS: Inhibition effect of drug-loaded nanoparticle on the proliferation of SMMC-7721 cells was determined with MTT method. Intra-cellular distribution of nanoparticles and morphological change of SMMC-7721 cells before and after treatment were observed by confocal laser scanning microscope (CLSM) and the apoptosis induced by adriamycin was measured by TUNEL assay. RESULTS: The inhibition effect of adriamycin-loaded chitosan nanoparticles surface-modified with glycyrrhizin on the proliferation of SMMC-7721 cells increased 6.36 times lower in comparison with free drugs. The inhibition effect of adriamycin-loaded chitosan nanoparticles had no obvious improvement. Nanoparticles surface-modified with glycyrrhizin promoted the distrubition of adriamycin in the nucleus, so as to promote the anti-tumor activity of adriamycin. TUNEL assay indicated that nanoparticles surface-modified with glycyrrhizin induce DNA fragmentation and nucleus breakdown to induce the apoptosis of SMMC-7721 cells. CONCLUSION: Adriamycin-loaded chitosan nanoparticles surface-modified with glycyrrhizin can used as potential active hepatocyte-targeted delivery carrier. Pharmacokinetic evaluation of it is worthy of further studing.
