Study on the Effects of Berberine on Colon Carcinoma in Vitro
- VernacularTitle:小檗碱对结肠癌的体外作用研究
- Author:
Ke WU
;
Junxia YANG
;
Qixin ZHOU
- Publication Type:Journal Article
- Keywords:
Aqueous extracts of Coloncar cinama;
Berberine;
Colon carcinoma;
COX-2
- From:
China Pharmacy
2005;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the inhibition effect of berberine on colon carcinoma lovo cell. METHODS:Colon car-cinoma lovo cells were cultured and treated with berberine and meloxicam in various concentrations. At 6 h,12 h and 24 h after treatment,the proliferation of lovo cell was assayed by MTT method. RT-PCR method and Western blot testing were used to eval-uate the mRNA and protein expressions of COX-2 in lovo cell. RESULTS:Berberine with concentration larger than 10-5 mol?L-1 and meloxicam with concentration larger than 10-4 mol?L-1 could inhibit the growth and proliferation of lovo cell and showed concentration and time dependent manners(P
