Inhibitory effect of EGCG on proliferation and HIF-1?/VEGF expression in cell line HepG2
- VernacularTitle:EGCG抑制肝癌细胞株HepG2增殖及HIF-1?/VEGF的表达
- Author:
Zhicheng ZHAO
;
Li ZHUANG
;
Changku JIA
;
Shusen ZHENG
- Publication Type:Journal Article
- Keywords:
Epigallocatechin gallate;
Liver neoplasms;
Hypoxia inducible factor-1;
Vascular endothelial growth factors
- From:Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the molecular mechanism of EGCG on inhibiting the growth of hepatic carcinoma. METHODS: The proliferation of hepatic cell line HepG2 cultured with different doses of EGCG was studied by MTT and suspension/adherence methods. The effect of EGCG on the expression of HIF-1?/VEGF at mRNA and protein levels in vitro and in vivo was evaluated by RT-PCR and Western blotting,respectively. The inhibition of EGCG on the growth of tumor implanted into athymic nude mice was also observed. RESULTS: The proliferation of hepatic cell line HepG2 was inhibited by EGCG in a dose-dependent manner. The expression of HIF-1?/VEGF was suppressed markedly by EGCG at protein level. However,the inhibitory effect of EGCG on the mRNA expression was only observed on VEGF,not on HIF-1?. In the animal experiment,the implanted tumor growth was inhibited by 39.8%?5.1%. CONCLUSION: EGCG suppresses the hepatic carcinoma cell growth,and interrupts the HIF-1?/VEGF signaling pathway significantly,indicating a fundamental mechanism of EGCG for inhibiting tumor growth.
