Determination of L-Carnosine in Polaprezine Granules by HPLC
- VernacularTitle:HPLC法测定聚普瑞锌颗粒中L-肌肽的含量
- Author:
Yali WANG
;
Hongling YU
;
Yajie BAO
- Publication Type:Journal Article
- Keywords:
HPLC;
Polaprezine granules;
L-Carnosine;
Content determination
- From:
China Pharmacy
1991;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop an HPLC method for the determination of L-Carnosine in Polaprezine granules.METHODS:The Chromatographic column was Agilent ODS with detection wavelength of 215 nm and flow rate of 1 mL?min-1.The mobile phase consisted of sodium subsulfite buffer solution(pH=3)-acetonetrile(77:23).The column temperature was set at 30 ℃ and injection size was 10 ?L.RESULTS:The good linearity was obtained in the range of 0.1559～3.1584 ?g for L-Carnosine(r=1.0000).The average recovery was 99.65%(RSD=0.078%).CONCLUSION:The method is simple,accurate and sensitive,and it is suitable for content determination of L-Carnosine in Polaprezine granules.
