Determination of Chlorogenic Acid in Ergan Oral Liquid by HPLC
- VernacularTitle:HPLC法测定儿感口服液中绿原酸的含量
- Author:
Fan JIANG
- Publication Type:Journal Article
- Keywords:
Ergan oral liquid;
Content determination;
Chlorogenic acid;
HPLC
- From:
China Pharmacy
2007;0(33):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an HPLC method for the determination of the content of chlorogenic acid in Ergan oral liquid.METHODS: The sample was determined on Scienhome C18(250 mm?4.6 mm,5 ?m) with mobile phase consisted of acetonitrile-0.4% phosphoric acid(12∶88) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 327 nm.RESULTS: The linear range of chlorogenic acid was 0.169 6～0.848 ?g(r=0.999 9) and the average recovery rate of chlorogenic acid was 98.88%(RSD=0.48%,n=6).CONCLUSION: The method is simple,accurate and reproducible,and it is applicable for the quality control of Ergan oral liquid.
