Effects of Compound Glycyrrhizin on Chemotactic Factors' Expression in Peripheral Blood Mononuclear Cells
- VernacularTitle:复方甘草酸苷对外周血单核细胞趋化因子表达的影响
- Author:
Jing FAN
;
Jinhua XU
- Publication Type:Journal Article
- Keywords:
Peripheral blood monouclear cells;
Compound glycyrrhizin;
Chemotatic factor;
Expression
- From:
China Pharmacy
2005;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study whether the therapeutic action of compound glycyrrhizin(SNMC) on allergic disorders can be achieved through inhibition on the expression of chemotactic factors in peripheral blood mononuclear cells(PBMC).METHODS: PBMC of 15 healthy subjects were obtained and divided into 4 drug-treated groups: SNMC group,dexamethasone group,mizolastine group,and loratadine group;meanwhile,a positive control group(proinflammatory stimulant was added) and a negative control group(no proinflammatory stimulant was added) were used as control.Fluorescent quantitation polymerase chain reaction was employed to determine the mRNA expression of the chemotatic factors including RANTES,MCP-1,MIP-1? and ELISA was employed to detect their concentrations in supernatant.RESULTS: In the positive control group,both the mRNA expressions and the concentrations of the 3 chemotatic factors increased significantly,and which were significantly lower in the drug-treatment groups than in the positive control group.There were significant differences between the positive control group and dexamethasone-treated group or SNMC-treated group in the expression of MIP-1? mRNA(P
