Effects of Resveratrol on SIRT1 Expression,eNOS Activity and NO Secretion of Hyperlipid Cultured HUVEC
- VernacularTitle:白藜芦醇对高脂处理人脐静脉内皮细胞SIRT1表达、eNOS活性及NO分泌的影响
- Author:
Juhong YANG
;
Nan WANG
;
Jingyan LI
;
Jiaojiao ZHANG
;
Ping FENG
- Publication Type:Journal Article
- Keywords:
Resveratrol;
Human umbilical vein endothelial cell;
SIRT1;
Hyperlipid;
Nitric oxide
- From:
China Pharmacy
2005;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the effects of resveratrol on the expression of SIRT1,the activity of eNOS and the secretion of NO of highlipid cultured primary human umbilical vein endothelial cells(HUVEC).METHODS: HUVEC were divided into normal control group,highlipid group and highlipid+resveratrol group,cultured for 24 hours.The expression of SIRT1 mRNA and protein were analyzed by RT-PCR and Western blot methods.Supernatant were used to analyze the nitric oxide(NO) content and activity of endothelial nitric oxide synthase(eNOS).RESULTS: Compared with highlipid group,resveratrol group increased the expression of SIRT1 mRNA and protein,the activity of eNOS and the secretion of NO(P
