Determination of Berberine Hydrochloride in Keyangmin Tincture by RP-HPLC
- VernacularTitle:RP-HPLC法测定克痒敏酊中盐酸小檗碱的含量
- Author:
Rong CHEN
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Berberine hydrochloride;
Keyangmin tincture;
Content determination
- From:
China Pharmacy
2005;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an RP-HPLC method for the determination of Berberine hydrochloride in Keyang-min tincture.METHODS:The sample separation was performed on Agilent Eclipse XDB C18(250 mm?4.6 mm,5 ?m),and the mobile phase was acetonitrile-5 mmol?L-1 perfluorooctane sulfonate(32∶68) at flow rate of 1 mL?min-1.The detection wavelength was 346 nm and the temperature of column was 35 ℃.RESULTS:The linear range of Berberine hydrochloride was 0.059 2～0.888 3 ?g(r=0.999 9).The average recovery rate was 99.71%(RSD=1.18%,n=6).CONCLUSION:The met-hod is sensitive,accurate,and reproducible,and suitable for the determination of Berberine hydrochloride in Keyangmin tincture.
