Relationship between delayed cardioprotection effect of 11,12-EET and phosphorylated ERK during ischemia and reperfusion in the rat myocardium
- VernacularTitle:11,12-EET的延迟性心脏保护作用与磷酸化ERK1/2的关系
- Author:
Hongxia WANG
;
Xiangjun ZENG
;
Ying JIANG
;
Lingqiao LU
;
Gang HAO
;
Yingfen ZHU
;
Like ZHANG
- Publication Type:Journal Article
- Keywords:
Myocardium;
Reperfusion injury;
Mitogen-activated protein kinases;
Epoxyeicosatrienoic acids
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: In order to study the relationship of the activation of ERK and delayed cardioprotection of 11,12-EET.METHODS: A rat ischemia/reperfusion(I/R) model was replicated by ligating left anterior descending coronary artery 30 min followed by 60 min.The expression of ERK was detected with Western blotting,and the change of heart function during reperfusion was observed.RESULTS: The difference of myocardial function was prominent at (24 h) in I/R group compared with sham group,EET+I/R and EET+PD098059+I/R group.The activity of ERK at(24 h) in EET+I/R group was higher than sham group, and the activity of ERK in EET+PD098059+I/R group was lower than that in EET+(I/R) group;the expression of phosphorylated ERK1/ERK2 at(24 h) in EET+I/R group was more than that in I/R group,and the expression of phosphorylated ERK1/ERK2 in EET+PD098059+I/R group was less than EET+I/R group.CONCLUSION: 11,12-EET has a delayed cardioprotection effect,and this protection effect is involved in the activity of ERK and expression of phosphorylated ERK1/ERK2.
