Activation of smad signaling and collagenⅠ synthesis in NRK52E cells induced by advanced glycation end products
- VernacularTitle:AGEs对肾小管上皮细胞转分化、1型胶原合成及smad信号通路的影响
- Author:
Liao SUN
;
Xueqing YU
;
Shenglang ZHU
;
Wenfang CHEN
;
Xiaoyan LI
;
Zhanjun JIA
;
Xin WANG
;
Xianrui DOU
;
Xiuqing DONG
;
Huili SUN
- Publication Type:Journal Article
- Keywords:
Glycosylation end products,advanced;
Collagen type Ⅰ;
Smad mRNA
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of advanced glycation end products on activation of Smad signaling pathway and collagenⅠ synthesis in proximal tubular epithelial cells.METHODS: Advanced glycation end products(AGE-BSA) were prepared by incubation of bovine serum albumin(BSA) with D-glucose.Normal rat proximal tubular epithelial(NRK52E) cells were cultured in RPMI-1640 medium with AGE-BSA.Phosphorylation and nuclear translocation of Smad2/3 were examined by immunocytochemistry.Levels of TGF-?_1 in supernatant of cell culture were measured by enzyme-linked immunosorbent assay(ELISA).Expression of TGF-?_1,Smad2,Smad3 and Smad7 mRNA were detected by RT-PCR.Expression of ?-SMA,E-cadherin and collagenⅠproteins were detected by Western blotting.RESULTS: AGE-BSA induced Smad2/3 phosphorylation and nuclear translocation,two peaks occured at 30 min(68% vs 16%,P
