Effects of advanced glycation end products on the expression of plasminogen activator inhibitor-1 in rat mesangial cells
- VernacularTitle:糖基化终末产物对大鼠肾系膜细胞纤溶酶原激活物抑制物-1表达的影响
- Author:
Xiaoyan YU
;
Cai LI
;
Chunsheng MIAO
;
Guihua ZHOU
;
Xiuyun ZHANG
- Publication Type:Journal Article
- Keywords:
Glomerular mesangium;
Glycosylation end products, advanced;
Plasminogen activator inhibitor-1;
Diabetic nephropathies
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of advanced glycation end products (AGEs) on the expression of plasminogen activator inhibitor-1 (PAI-1) in rat mesangial cells and its relationship with extracellular matrix accumulation. METHODS: Rat mesangial cells were treated with AGE-modified bovine serum albumin or native bovine serum albumin. Normal mesangial cells without any treatments were used as control. Fibronectin (FN), collagen Ⅳ, PAI-1 protein contents were detected by ELISA. PAI-1 mRNA was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: AGEs (0-200 mg/L) did not influence mesangial cells proliferation, but stimulated FN , collagen IV and PAI-1 contents in mesangial cell cultured medium in different degrees. AGEs also increased PAI-1 mRNA expression. CONCLUSION: AGEs increase the expression of PAI-1 in rat mesangial cells. AGEs may reduce ECM degradation through increasing PAI-1 expression, which may be one of the mechanisms of ECM accumulation in diabetic nephropathy.
