The cardioprotective effect of the inhibitor of JAK/STAT on simulating ischemia-reperfusion injury in cultured immature rat cardiacmyocytes
- VernacularTitle:抑制JAK/STAT通路对培养乳鼠心肌细胞模拟缺血/再灌注损伤的作用
- Author:
Cheng SHEN
;
Shizhi FAN
;
Jianming CHEN
;
Zhiping LI
- Publication Type:Journal Article
- Keywords:
Myocardial reperfusion injury;
Transcription initiation site;
Signal transduction
- From:
Journal of Chinese Physician
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the cardioprotective effects of the inhibitor of JAK/STAT pathway on ischemia-reperfusion injury in cultured immature rat cardiacmyocytes.Methods The cultured immature rat cardiacmyocytes were randomly divided into normal control group,ischemia-reperfusion group,AG490 treatment group and RMP treatment group.The cell viability was measured by MTT method.The contents of lactate dehydrogenase(LDH) and malondialdehyde(MDA),the superoxide dismutase(SOD) activity and the rate of cell apoptosis were detected.Results Compared with the normal group,the cell viability decreased significantly,the contents of LDH and MDA in the supernatant of the cells increased significantly,the SOD activity decreased and the rate of apoptosis of cells increased significantly in ischemia-reperfusion group(all P
