Role of p38MAPK activation in high glucose-induced collagen Ⅲ synthesis in normal rat kidney tubular epithelial cells NRK52E
- VernacularTitle:p38MAPK参与高糖刺激大鼠肾小管上皮细胞产生细胞外基质胶原Ⅲ
- Author:
Xiaoli JING
;
Dongping WANG
;
Xiaoxing LIAO
;
Jinhua LI
;
Xin LI
- Publication Type:Journal Article
- Keywords:
Glucose solution, hypertonic;
Diabetes mellitus;
Kidney interstital fibrosis;
p38MAP kinase;
Extracellular matrix collagen Ⅲ
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the role of p38 mitogen-activated protein kinase (p38MAPK) activation in high glucose-induced collagen Ⅲ synthesis in NRK52E cells. METHODS: Normal rat tubular epithelial cell line NRK52E was cultured in D-glucose of different concentrations, pretreated with SB203580 and collected at different time points. The levels of phospho-p38MAPK and extracellular matrix collagen Ⅲ were examined by Western blotting. RESULTS: The activation of p38MAPK was shown to be dependent upon D-glucose concentration and the time-course. Pretreatment with SB203580 blocked p38MAPK activation induced by high concentration of D-glucose in NRK52E cells. CONCLUSIONS: The activation of p38MAPK induced by high concentration of glucose may play a role in diabetic interstital renal fibrosis. SB203580 has a potential value of clinical applications in the prevention and treatment of diabetic nephropathy.
