Determination of the Content of Berberine Hydrochloride in the Fukezhidai Tablets by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定妇科止带片中盐酸小檗碱的含量
- Author:
Guizhen TENG
- Publication Type:Journal Article
- Keywords:
Fukezhidai tablet;
berberine hydrochloride;
RP-HPLC
- From:
China Pharmacy
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a RP-HPLC method for determination of the content of berberine hydrochloride in the Fukezhidai tablets METHODS:HPLC was performed on Dimonsil C18 column with CH3CN-KH2PO4(30∶70)as mobile phase The detection wavelength was 347nm RESULTS:The linear range of berberine on calibration curve was 0 4 052～2 026?g/ml The average recovery of berberine was 98 18%,RSD=0 92%(n=5) CONCLUSION:The method was simple and accurate,and can be used for the quality control of Fukezhidai tablets
