Effects of advanced glycation end products on protein and mRNA expression of macrophage inflammatory protein-1? in cultured human umbilical vein endothelial cells
- VernacularTitle:糖基化终产物对培养的人脐静脉内皮细胞巨噬细胞炎性蛋白-1?表达的影响
- Author:
Xin MENG
;
Jin ZHANG
;
Wei WU
;
Song BAI
- Publication Type:Journal Article
- Keywords:
Glycation end products, advanced;
Endothelium, vascular;
Macrophage inflammatory protein-1
- From:Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of advanced glycation end products (AGEs) on protein and mRNA expression of macrophage inflammatory protein-1? (MIP-1?) in cultured human umbilical vein endothelial cells(HUVECs). METHODS: HUVECs were cultured with AGEs at different concentrations for 24 h and at a concentration of 400 mg/L for different time.The levels of mRNA and protein expression of MIP-1? in cultured HUVEC were detected by in situ hybridization and Western blot, respectively. RESULTS: In situ hybridization showed that after exposure of HUVECs to AGEs at different concentrations (100 mg/L, 200 mg/L, 400 mg/L) for 24 h, the average integrated optical density values (18.76?3.17, 26.58?1.61, 34.23?2.25) of MIP-1? mRNA expression in HUVECs were higher than that in control group (13.83?1.24, P
