A study on p38MAPK activity during mitomycin-induced apoptosis of cholangiocarcinoma cell lines
- VernacularTitle:丝裂霉素诱导胆管癌细胞凋亡过程中p38MAPK表达的研究
- Author:
Bo WANG
;
Xueguang ZHU
;
Jun LI
;
Hua ZHAO
;
Na ZHAO
- Publication Type:Journal Article
- Keywords:
Cholangiocarcinoma;
Protein kinases;
Mitomycins;
Apoptosis
- From:
Chinese Journal of General Surgery
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of p38MAPK activity during mitomycin-induced cell cycle arrest of cholangiocarcinoma. Using p38MAPK inhibitor SB203580 to analyze the effect on chemotherapy of cholangiocarcinoma. Methods The selected human cholangicarcinoma cell lines RBE was treated with mitomycin or together with SB203580, cell apoptosis was analysed by AnnexinV-FITC and propidium iodide(PI)double staining and flow cytometry (FCM), p38 and ATF-2 activity was detected by Western blot. Results Mitomycin induced apoptosis of RBE cell line, the ratio of apoptosis was (8.91?0.83)%( P
