A study on insulin receptor on human hepatocellular cancer cell membrane
- VernacularTitle:人肝癌细胞膜胰岛素受体的研究及临床意义
- Author:
Zhong CHENG
;
Xiaohong OU
;
Anren KUANG
- Publication Type:Journal Article
- Keywords:
Receptors, insulin;
Carcinoma,hepatocellular
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the insulin receptor expression binding characteristics and it's clinical significance of 125 I insulin on human hepatocllular cancer cell membrane.MethodInsulin was radioiodinated using Ch T method, isolated and purified by polyacrylamide gel electryophresis. Human hepatocellular cancer and adjacent liver tissue cell membrane were extracted, receptor binding and bind ability of 125 I insulin and insulin receptor on hepatocellular cancer and adjacent hepatic cell membrane were studied. ResultsThe specific activity of 125 I insulin was 46 1?1 2 TBq/mmol,the radiochemical purity was over 98%. Insulin receptor content increased in hepatocellular carcinoma cells 〔Bmax=(1 9?0 6)?pmol/mg protein, the Kd of high and low affinity was 2 1?0 6?nmol and 25?8?nmol respectively〕 compared with carcinoma adjacent hepatic cells 〔Bmax=( 1 42? 0 57)?pmol/mg protein t =0 032 P 0 05〕.ConclusionHuman hepatocellular carcinoma cells overexpress insulin receptor. A14 125 I insulin has a specific high affinity with insulin receptor on HCC cells.
