Determination of polymethoxylated flavones in Citri Reticulatae Pericarpium from eleven cultivar origins
10.3969/j.issn.1001-1528.2017.03.026
- VernacularTitle:11个品种来源陈皮中多甲氧基黄酮的测定
- Author:
Hujie LUO
;
Yiting YANG
;
Xiaowei LI
;
Meixia LUO
;
Pingjun HU
;
Hao CHEN
;
Guodong ZHENG
- Keywords:
Citri Reticulatae Pericarpium;
cultivar origins;
polymethoxylated flavones;
ultraviolet-visible spectrophotometry
- From:
Chinese Traditional Patent Medicine
2017;39(3):565-569
- CountryChina
- Language:Chinese
-
Abstract:
AIM To determine the contents of polymethoxylated flavones in Citri Reticulatae Pericarpium from eleven cultivar origins.METHODS HPLC was applied to screening extraction solvents (methanol,anhydrous ethanol,95％ ethanol,ethyl acetate and petroleum ether).Ultraviolet-visible spectrophotometry was adopted in the content determination of polymethoxylated flavones in eighteen batches of samples,nobiletin was taken as a reference substance,and the detection wavelength was set at 330 nm.RESULTS Ethyl acetate exhibited the best extraction effect,so it was selected as extraction solvent.Nobiletin showed a good linear relationship within the range of 3.024-13.104 μg/mL (R2 =0.999 8),whose average recovery was 101.37％ with the RSD of 1.34％.The contents of polymethoxylated flavones had obvious differences among samples from different cultivar origins,which were relatively high from Citrus reticulata ‘ Chachi’,Citrus reticulata ‘ Dahongpao’,Citrus reticulata ‘ Tangerina’,Citrus reticulata ‘ Ponkan’,Citrus reticulata ‘ Kinokuni’and Citrus reticulata ‘ Shiyueju’(more than 0.4％).CONCLUSION This simple and accurate method can effectively eliminate the interference of another flavonoids,which is suitable for the content determination of polymethoxylated flavones in Citri Reticulatae Pericarpium.
